Bayanmunkh A
Drug Research Institute, Mongolia
Title: Content of Ginsenoside Rg1 in the Panax ginseng cultivated in Mongolia
Biography
Biography: Bayanmunkh A
Abstract
The Panax ginseng is one of the most important medicinal plants in Asia. It has not been grown in Mongolia. Since 2014, we have been trying to cultivate Panax ginseng in Mongolian Gobi desert. The saponins such as ginsenosides are the main bioactive compounds in P. ginseng. The present study investigated the growth characteristics of Ginsenoside Rg1 content in roots of Panax ginseng at different cultivars (from 1 and 5 years). Minimum 0.40% for the sum of ginsenosides Rg1 and Rb1 in the Panax ginseng is standardized in the British and European pharmacopeia articles. The purpose of this study is to describe Panax ginseng is possible to cultivate in Mongolia and to determine which aged cultivar is richest content of ginsenoside. Roots of different aged of P. ginseng were collected at October of 2015 in field of Umnugovi province, Mongolia as studying plants. Collected samples were dried and powdered. Samples were extracted with 70% aqueous methanol. The extract was filtrated through filter paper (Whatman No. 42) and evaporated vacuum rotor. A Shimadzu LC-20AD liquid chromatograph equipped with quaternary gradient pump and extracted as described above. For comparison, a manual sampler and UV-Vis detection unknown sample was concurrently prepared and system was used. A HPLC method was developed. Separation was carried out using a reversed-phase column LiChrosorb® RP-18 (250*4.5 mm I.D., 5 µm). The binary gradient elution system consisted of water (adjusted to pH 2 with phosphoric acid) (A) and acetonitrile (B). The Panax ginseng was successfully cultivated in Mongolian Gobi desert. Also the following result is determined contents of Rg1 ginsenoside: 1 aged root, 2.03%; 2 aged root, 2.15%; 4 aged root, 2.31% and 5 aged root, 0.26%. The Mongolian ginseng root had the highest content of ginsenosides Rg1 in 4 aged roots and decreased in next years.