Day 1 :
USC School of Pharmacy , USA
Time : 10:00-11:00
James Adams was trained for 14 years by a California Indian Healer, Chumash. He has used these medicines to treat many patients over the years. He has also investigated the photochemistry of California medicinal plants and has published several articles on the subject. He teaches Chumash Healing to pharmacy and medical students and to the public.
Statement of the Problem: The use of oral NSAIDs and opioids kills patients by causing strokes (80,000 resulting in 14,000 deaths), heart attacks (75,000 resulting in 12,000 deaths), ulcers (100,000 resulting in 10,000 deaths), respiratory depression (>18,000 deaths), kidney toxicity and seizures. It is estimated that 54,000 or more people in the US die every year from these dangerous drugs. The US has a fixation with using these dangerous oral drugs. Many clinicians are unable to imagine any other way to treat pain.
Methodology & Theoretical Orientation: Liniments are available that treat even broken bone pain and other severe pain. Liniments are available that cure chronic pain such as fibromyalgia, whiplash and chronic back pain. Acupuncture successfully treats pain in many patients. Heat or ice therapy is effective for many pain patients.
Findings: Liniments that contain monoterpenoids provide very effective pain relief, even for severe pain. The use of single monoterpenoids is not effective. Multiple monoterpenoids should be present to inhibit multiple types of transient receptor potential cation channels in the skin. These receptors are the major pain receptors and are highly concentrated on skin sensory afferent neurons. Sesquiterpenes should also be present in the liniment to inhibit COX-2 activity and expression. COX-2 is up-regulated in the skin in chronic pain conditions. COX-2 makes prostaglandins that cause and magnify pain. Monoterpenoids and sesquiterpenes cross the skin and are effective when applied topically.
Conclusion & Significance: Pain is most effectively and safely treated in the skin. The use of acupuncture is very effective and safe. The application of small amounts of liniment at painful sites on the skin is effective and avoids systemic toxicity. Liniments that down regulate COX-2 can cure chronic pain.
Foamix Pharmaceutical Ltd, Isreal
Time : 11:20-12:20
Dov Tamarkin is the Chief Executive Officer of Foamix Pharmaceuticals Ltd., and Co-Inventor of the Foamix Foam technology. He has 30 years of pharmaceutical experience where he held multiple senior management positions at Teva Pharmaceuticals, Portman Pharmaceutical Industries, PowerPaper and TPI. He has a PhD degree in Chemistry from The Hebrew University of Jerusalem, Israel and is the Inventor of over a 100 patents in the pharmaceutical field.
Foam is becoming a prominent delivery system for topical drugs. This platform provides an innovative, easy to apply, novel alternative for creams and ointments. To-date, all foams are collectively designated as Medicated Foams by the European Pharmacopoeia and as Foam Aerosol being a sub-part of the aerosol section in the US Pharmacopoeia. Not all foams are equal. While in the past there were few types of medicated foam i.e., aqueous foams and hydro-ethanolic foams, today there are several types of foam formulations being marketed and additional types under development, which differ from each other by their composition and functionality. For example, an emulsion-based foam, which is the equivalent of a cream; a petrolatum-based foam which is the equivalent of an ointment; a hydrophilic solvent based foam comprising PEG or propylene glycol, which is the equivalent of a hydrophilic ointment and an oil-based foam which is the equivalent of an oil solution or suspension. It would be of importance for the pharmaceutical scientist, as well as for the practicing physician, to understand the difference between the above-mentioned types of foam formulations and to be able to select the appropriate type of foam formulation for a given clinical condition. The current presentation will introduce the Rosetta stone of foams. It will describe the types of foams and provide a functional translation to their respective traditional topical dosage forms. The presentation will further display examples of unique dermal and transdermal drug delivery profiles, attained by foam formulations. By attenuating the foam composition, one can achieve intradermal or transdermal delivery preference. The presentation will further provide unique results from clinical trials that evaluated the efficacy of the first-in-class Minocycline Foam, in the treatment of moderate-to-severe acne and rosacea.
- Drug Design And Development | Computer Aided Drug Design (CADD) | Pharmacology and Toxicology | Traditional Medicine Design | Drug Formulation Procedures| Innovative Drug Discovery and Nanotechnology
Al-Azhar University, Egypt
He is the professor at Al-Azhar University, Egypt, and he worked at Nile trade Company
Bifidobacterium represent one of the major genera of the intestinal tract of human and animals used as probiotics in dairy and non-dairy foods for restoring the intestinal microflora which confers a health benefit. The identification of Bifidobacterium by phenotypic features is commonly unreliable, time, money and effort consuming. We sought to improve the Bifidobacterium identification method based on molecular level to identify probiotic bacteria in complex microbial communities. The application of 16S-23S rRNA oligonucleotide primers is the best and most reliable, rapid and precise species and sub species identification approach. The ribosomal intergenic spacer region (ISR) located between the highly conserved 16S rRNA and 23S rRNA shows a high degree of variation in length and sequence and potential for intra species discrimination and providing the phylogenetic relationship of the Genus Bifidobacterium spp. Results showed that one of the two primer sets Bflac2-Bflac5 species specific gives positive results differentiating between B. animalis ssp. lactis isolated from breast fed infants milk of human and that isolated from feces of breast fed infant and detecting reference strain for B. animalis ssp. lactis DSM10140. DNA sequences of the two strains were submitted to the Genbank NCBI under accession number (KT758845) named as B. animalis ssp. lactis Egm1 (Egyptian milk) and accession number (KT758846) named as Egf1 Egyptian feces while the second primer give false positive result. Also, we aim to obtain patent protection under Intellectual Property Rights (IPRs) for B. animalis ssp. lactis which was isolated from Egyptian resources to be used for a better and healthier food and dairy products.
Medical University of Warsaw, Poland
Maciej Malecki, PhD, Prof, is working at the Department of Applied Pharmacy in the Medical University of Warsaw, Poland. He is the author of about 100 publications in the field of medical biology and pharmacy. He is interested in cancer gene therapy and rAAV vectorology.
Introduction: Melanoma is a cutaneous cancer characterized by the highest mortality rates and a malignancy with the highest potential of dissemination. The prognosis of patients with metastatic melanoma is poor. Treatment proposed so far has not produced beneficial effects. Therefore, one of the current therapeutic approaches to melanoma is gene therapy with the use of recombinant adeno-associated viruses (rAAV) as gene vectors. The rAAV vectors are the principal candidates for virus-based gene therapy because of their small size, broad tissue tropism, safety profile and low immunogenicity. The purpose of this study involved a targeted delivery of rAAV 2/2 and 2/6 formulations to melanoma cells metastasized into murine lungs.
Methodology: In our experiments, we used intranasal (in) and intraperitoneal rAAV (ip) formulations of serotype 2/2 and 2/6 encoding Gfp reporter under the control of cmv promoter. The experiments were performed in vivo on B16-F10 melanoma tumor-bearing C57BL/6 mice. The mice were injected with B16-F10 tumor cells via the tail vein. After 14 days the rAAV vectors were administered to the mice by in or ip injection, and 7 days later the mice were euthanized, and the infected tissues collected for further tests. The infection efficiency of melanoma cells metastasized into murine lungs was assessed with qPCR method. At the same time, we performed the analysis of the bio-distribution of rAAV vectors to different organs in mice. Conclusion: The study demonstrated the usefulness of rAAV formulations in introducing genes into metastatic melanoma cells. The highest infection efficiency was observed for serotype 2 after intraperitoneal injection. Gene therapy with the use of rAAV formulations may be a promising therapeutic strategy for melanoma and lung cancer in the future. This work was supported by a grant from NCBiR (Strategmed1/233264/4/NCBR/2014, MentorEYE).
Drug Research Institute, Mongolia
The Panax ginseng is one of the most important medicinal plants in Asia. It has not been grown in Mongolia. Since 2014, we have been trying to cultivate Panax ginseng in Mongolian Gobi desert. The saponins such as ginsenosides are the main bioactive compounds in P. ginseng. The present study investigated the growth characteristics of Ginsenoside Rg1 content in roots of Panax ginseng at different cultivars (from 1 and 5 years). Minimum 0.40% for the sum of ginsenosides Rg1 and Rb1 in the Panax ginseng is standardized in the British and European pharmacopeia articles. The purpose of this study is to describe Panax ginseng is possible to cultivate in Mongolia and to determine which aged cultivar is richest content of ginsenoside. Roots of different aged of P. ginseng were collected at October of 2015 in field of Umnugovi province, Mongolia as studying plants. Collected samples were dried and powdered. Samples were extracted with 70% aqueous methanol. The extract was filtrated through filter paper (Whatman No. 42) and evaporated vacuum rotor. A Shimadzu LC-20AD liquid chromatograph equipped with quaternary gradient pump and extracted as described above. For comparison, a manual sampler and UV-Vis detection unknown sample was concurrently prepared and system was used. A HPLC method was developed. Separation was carried out using a reversed-phase column LiChrosorb® RP-18 (250*4.5 mm I.D., 5 µm). The binary gradient elution system consisted of water (adjusted to pH 2 with phosphoric acid) (A) and acetonitrile (B). The Panax ginseng was successfully cultivated in Mongolian Gobi desert. Also the following result is determined contents of Rg1 ginsenoside: 1 aged root, 2.03%; 2 aged root, 2.15%; 4 aged root, 2.31% and 5 aged root, 0.26%. The Mongolian ginseng root had the highest content of ginsenosides Rg1 in 4 aged roots and decreased in next years.
Al-Azhar University, Egypt
Benzo[a]pyrene induced lung cancer by mechanism which interact with DNA and cause genetic changes; this mechanism accelerates the cell cycle progression and induces the abnormal cell proliferation. Selenium, N Acetyl cysteine and curcumin in nanocomposite have been shown to confer various biological effects, anticancer, enhance immune system and antioxidant properties. The present study was undertaken to evaluate the chemopreventive effect of nano (selenium ,acetl cysteine ,curcumin) (NSACC) and possess ability of SNACC with dose 4mg kg.b.w against Benzo[a]pyrene carcinogenesis with dose 200mgkg.bw divided at two doses the first at the 1st week of the experiment the second after 20 week from the time of the experiment .The results indicated that B[a]p induced lung cancer in mice's histopathologically and cause significant decrease of SOD, GSH,CAT values and significant increase of NOx, LP over expression of p53,cas3 and cas9.While, treating with(NSACC) causes significant increase of SOD, GSH, GPx, and significant decrease of CAT, LP, Nox, induction of p53, cas 3, cas 9 gradually then decrease to normal control values. From the obtained results, it could be concluded that inhibition of peroxidation and oxidative stress markers, enhanced antioxidant status, induction of p53 expression , caspase3 and 9 gene in mice lung tissue by NSACC suggest the potential efficacy of NSACC as an addition chemo preventive agent in treatment of lung carcinogenesis. These data provide direct evidence for the role of NSACC as very strong chemo preventive and treating drug for lung cancer induced by B[a]P.