Day 2 :
- Pre-Formulation & Formulation Aspects | Pharmacokinetics and Pharmacodynamics | Novel Drug Delivery System
Location: 2
Session Introduction
Hytham E Shoura
AL-Azhar University, Egypt
Title: Isolation of mstn gene As A candidate gene of the muscles growth
Biography:
Abstract:
Myostatin is a transforming growth factor-b (TGF-b) family member that plays an essential role in regulating skeletal muscle growth. It is expressed initially in the myotome compartment of developing somites and continues to be expressed in the myogenic lineage throughout development and in adult animals. Individual muscles of myostatin null weigh approximately twice as much as those of wild type mice as a result of a combination of muscle fiber hyperplasia and hypertrophy. The myostatin sequence and function have been highly conserved through evolution . Remarkably, the human, rat, murine, porcine, goat, turkey, and chicken myostatin sequences are identical in the biologically active C-terminal portion of the molecule following the proteolytic processing site, , These findings have raised the possibility that pharmacological agents capable of blocking myostatin activity may have applications for promoting muscle growth in human disease settings as well as in livestock animals using gene therapy. our conducted study focused on isolating and identifying the Myostatin gene (MSTN) as a candidate gene of the muscles growth trait in the Egyptian goat breeds (zaraibi, baladi, Damascus), samples were obtained from the Egyptian National Gene Bank, three pairs of primers were designed to amplify MSTN gene, the sequences fragments were aligned with the NCBI database using BLASTN tool to find the high similar sequences, the results showed that there are high similarity between the sequenced fragments and Myostatin gene sequence of (sheep, bovine, camel, chicken, human)in the database, DNA fragments have been sequenced and submitted to the NCBI database under KY441464, KY463684, KY463685 and KY463686 accession numbers for MSTN gene partial sequences from Egyptian goat breeds.
Mohmed Elrodini
Ministry of Health and Population & Nile Trade Co, Egypt
Title: Molecular pathogenesis of gastric ulcers and strategies for prevention
Biography:
Abstract:
The purpose of this review is to summarize the pertinent literature published in the present era regarding ulcerogenic effectors, and all available therapeutic concepts in this regard including; different physiological/pathological changes in response to H. pylori infection, nonsteroidal anti-inflammatory drugs (NSAID), bile acids, nitric oxide, copper complexes, acid pump inhibitors, histamine blockers, curcuminoids, cytokines and/or growth factors and finally probiotics. Because of the partial understanding of gastric ulcer pathogenesis three major hypotheses were strongly speculated and widely documented. Firstly, the hyperacidity hypothesis entailing the disturbance of the gastric acid, histamine, gastrin and somatostatin. Secondly, the eicosanoid imbalance hypothesis exploiting changes in the microcirculation through the vasoconstrictor eicosanoids such as TXA2 and vasodilator cytoprotectant eicosanoids such as PGE2. Thirdly, the infective hypothesis implementing H. pylori as the major pathogenic effectror for the gastroduodenal ulceration. In fact, all of the previous effectors could be involved and possibly employing inflammatory/antiinflammatory, oxidative stress and/or angiogenic disturbance.
Ahmed R Awaad
Al - Alzhar University, Egypt
Title: Effects of some lactobacillus strains on plasma lipids of rats and fecal microflora
Biography:
Abstract:
Lactobacillus represent one of the major genera of the intestinal tract of human and animals and are used, as probiotics, in dairy and non-dairy foods to restore the intestinal microflora which confer a health benefit. After an adaptation period for 7 days, the first group was fed on basal diet (80 g- for each rat group /day) and served as control I, while the second group was offered basal diet plus standardized buffalo's milk (40 ml. for each rat group / day) and served as control II. The other groups were fed on 80 grams of basal diet for each rat group / day and 40 ml. / day for each rat group, buffalo's milk plus one of the following Lactobacillus strains respectively L.casei strain AZ1, L.rhamnosus strain AZ1 and L.gasseri strain AZ1. Furthermore, supplementation of diets with fermented milk products cultured with L. casei KY123805 or L. rhamnosus KY123789 resulted in noticeable decreases in Total cholesterol, HDL- cholesterol and triglycerides levels at the end of the experiment (28 days) as compared to dry diet (control I). Species of lactobacilli occurring in intestinal tract deconjugate both taurocholic and glycocholic acids, such serum cholesterol levels when it is considered that deconjugated bile acids function more poorly in supporting adsorption of lipids from the intestinal tract than deconjugated ones, this could result in reduce adsorption of cholesterol from the intestines and thus influence its serum level. Therefore, the main target of the present investigation was to isolate and identify some local isolates belonging to genera Lactobacillus. Also, the isolated strains have been screened in order to define their characteristics that would be as probiotic strains or not. Furthermore, the long-term goal of this work is to registering patent protection for some Lactobacillus spp. isolated from local Egyptian resources to increase the additive values of the Egyptian microbial wealth and well use it in the industrial healthy dairy products and pharmaceutical.