9^th Annual Congress on Drug Design & Drug Formulation October 19-20, 2017 Seoul, South Korea

Biography:

He is the professor at Al-Azhar University, Egypt, and he worked at Nile trade Company 

Abstract:

Biography:

Maciej Malecki, PhD, Prof, is working at the Department of Applied Pharmacy in the Medical University of Warsaw, Poland. He is the author of about 100 publications in the field of medical biology and pharmacy. He is interested in cancer gene therapy and rAAV vectorology.

Abstract:

Introduction: Melanoma is a cutaneous cancer characterized by the highest mortality rates and a malignancy with the highest potential of dissemination. The prognosis of patients with metastatic melanoma is poor. Treatment proposed so far has not produced beneficial effects. Therefore, one of the current therapeutic approaches to melanoma is gene therapy with the use of recombinant adeno-associated viruses (rAAV) as gene vectors. The rAAV vectors are the principal candidates for virus-based gene therapy because of their small size, broad tissue tropism, safety profile and low immunogenicity. The purpose of this study involved a targeted delivery of rAAV 2/2 and 2/6 formulations to melanoma cells metastasized into murine lungs.

Methodology: In our experiments, we used intranasal (in) and intraperitoneal rAAV (ip) formulations of serotype 2/2 and 2/6 encoding Gfp reporter under the control of cmv promoter. The experiments were performed in vivo on B16-F10 melanoma tumor-bearing C57BL/6 mice. The mice were injected with B16-F10 tumor cells via the tail vein. After 14 days the rAAV vectors were administered to the mice by in or ip injection, and 7 days later the mice were euthanized, and the infected tissues collected for further tests. The infection efficiency of melanoma cells metastasized into murine lungs was assessed with qPCR method. At the same time, we performed the analysis of the bio-distribution of rAAV vectors to different organs in mice. Conclusion: The study demonstrated the usefulness of rAAV formulations in introducing genes into metastatic melanoma cells. The highest infection efficiency was observed for serotype 2 after intraperitoneal injection. Gene therapy with the use of rAAV formulations may be a promising therapeutic strategy for melanoma and lung cancer in the future. This work was supported by a grant from NCBiR (Strategmed1/233264/4/NCBR/2014, MentorEYE).

Biography:

Bayaraa Sukhbaatar is a Chemist, involved in the research projects on development of drug studies and Mongolian national standardization of drug technical requirements.

Abstract:

The Panax ginseng is one of the most important medicinal plants in Asia. It has not been grown in Mongolia. Since 2014, we have been trying to cultivate Panax ginseng in Mongolian Gobi desert. The saponins such as ginsenosides are the main bioactive compounds in P. ginseng. The present study investigated the growth characteristics of Ginsenoside Rg1 content in roots of Panax ginseng at different cultivars (from 1 and 5 years). Minimum 0.40% for the sum of ginsenosides Rg1 and Rb1 in the Panax ginseng is standardized in the British and European pharmacopeia articles. The purpose of this study is to describe Panax ginseng is possible to cultivate in Mongolia and to determine which aged cultivar is richest content of ginsenoside. Roots of different aged of P. ginseng were collected at October of 2015 in field of Umnugovi province, Mongolia as studying plants. Collected samples were dried and powdered. Samples were extracted with 70% aqueous methanol. The extract was filtrated through filter paper (Whatman No. 42) and evaporated vacuum rotor. A Shimadzu LC-20AD liquid chromatograph equipped with quaternary gradient pump and extracted as described above. For comparison, a manual sampler and UV-Vis detection unknown sample was concurrently prepared and system was used. A HPLC method was developed. Separation was carried out using a reversed-phase column LiChrosorb® RP-18 (250*4.5 mm I.D., 5 µm). The binary gradient elution system consisted of water (adjusted to pH 2 with phosphoric acid) (A) and acetonitrile (B). The Panax ginseng was successfully cultivated in Mongolian Gobi desert. Also the following result is determined contents of Rg1 ginsenoside: 1 aged root, 2.03%; 2 aged root, 2.15%; 4 aged root, 2.31% and 5 aged root, 0.26%. The Mongolian ginseng root had the highest content of ginsenosides Rg1 in 4 aged roots and decreased in next years.

Biography:

Abstract:

Benzo[a]pyrene induced lung cancer by mechanism which interact with DNA and cause genetic changes; this mechanism accelerates the cell cycle progression and induces the abnormal cell proliferation. Selenium, N Acetyl cysteine and curcumin in nanocomposite have been shown to confer various biological effects, anticancer, enhance immune system and antioxidant properties. The present study was undertaken to evaluate the chemopreventive effect of nano (selenium ,acetl cysteine ,curcumin) (NSACC) and possess ability of SNACC with dose 4mg kg.b.w against Benzo[a]pyrene carcinogenesis with dose 200mgkg.bw divided at two doses the first at the 1st week of the experiment the second after 20 week from the time of the experiment .The results indicated that B[a]p induced lung cancer in mice's histopathologically and cause significant decrease of SOD, GSH,CAT values and significant increase of NOx, LP over expression of p53,cas3 and cas9.While, treating with(NSACC) causes significant increase of SOD, GSH, GPx, and significant decrease of CAT, LP, Nox, induction of p53, cas 3, cas 9 gradually then decrease to normal control values. From the obtained results, it could be concluded that inhibition of peroxidation and oxidative stress markers, enhanced antioxidant status, induction of p53 expression , caspase3 and 9 gene in mice lung tissue by NSACC suggest the potential efficacy of NSACC as an addition chemo preventive agent in treatment of lung carcinogenesis. These data provide direct evidence for the role of NSACC as very strong chemo preventive and treating drug for lung cancer induced by B[a]P.

Biography:

Abstract:

Myostatin is a transforming growth factor-b (TGF-b) family member that plays an essential role in regulating skeletal muscle growth. It is expressed initially in the myotome compartment of developing somites and continues to be expressed in the myogenic lineage throughout development and in adult animals. Individual muscles of myostatin null weigh approximately twice as much as those of wild type mice as a result of a combination of muscle fiber hyperplasia and hypertrophy. The myostatin sequence and function have been highly conserved through evolution . Remarkably, the human, rat, murine, porcine, goat, turkey, and chicken myostatin sequences are identical in the biologically active C-terminal portion of the molecule following the proteolytic processing site, , These findings have raised the possibility that pharmacological agents capable of blocking myostatin activity may have applications for promoting muscle growth in human disease settings as well as in livestock animals using gene therapy. our conducted study focused on isolating and identifying the Myostatin gene (MSTN) as a candidate gene of the muscles growth trait in the Egyptian goat breeds (zaraibi, baladi, Damascus), samples were obtained from the Egyptian National Gene Bank, three pairs of primers were designed to amplify MSTN gene, the sequences fragments were aligned with the NCBI database using BLASTN tool to find the high similar sequences, the results showed that there are high similarity between the sequenced fragments and Myostatin gene sequence of (sheep, bovine, camel, chicken, human)in the database, DNA fragments have been sequenced and submitted to the NCBI database under KY441464, KY463684, KY463685 and KY463686 accession numbers for MSTN gene partial sequences from Egyptian goat breeds.

Biography:

Abstract:

The purpose of this review is to summarize the pertinent literature published in the present era regarding ulcerogenic effectors, and all available therapeutic concepts in this regard including; different physiological/pathological changes in response to H. pylori infection, nonsteroidal anti-inflammatory drugs (NSAID), bile acids, nitric oxide, copper complexes, acid pump inhibitors, histamine blockers, curcuminoids, cytokines and/or growth factors and finally probiotics. Because of the partial understanding of gastric ulcer pathogenesis three major hypotheses were strongly speculated and widely documented. Firstly, the hyperacidity hypothesis entailing the disturbance of the gastric acid, histamine, gastrin and somatostatin. Secondly, the eicosanoid imbalance hypothesis exploiting changes in the microcirculation through the vasoconstrictor eicosanoids such as TXA2 and vasodilator cytoprotectant eicosanoids such as PGE2. Thirdly, the infective hypothesis implementing H. pylori as the major pathogenic effectror for the gastroduodenal ulceration. In fact, all of the previous effectors could be involved and possibly employing inflammatory/antiinflammatory, oxidative stress and/or angiogenic disturbance.

Biography:

Abstract:

Lactobacillus represent one of the major genera of the intestinal tract of human and animals and are used, as probiotics, in dairy and non-dairy foods to restore the intestinal microflora which confer a health benefit. After an adaptation period for 7 days, the first group was fed on basal diet (80 g- for each rat group /day) and served as control I, while the second group was offered basal diet plus standardized buffalo's milk (40 ml. for each rat group / day) and served as control II. The other groups were fed on 80 grams of basal diet for each rat group / day and 40 ml. / day for each rat group, buffalo's milk plus one of the following Lactobacillus strains respectively L.casei strain AZ1, L.rhamnosus strain AZ1 and L.gasseri strain AZ1. Furthermore, supplementation of diets with fermented milk products cultured with L. casei KY123805 or L. rhamnosus KY123789 resulted in noticeable decreases in Total cholesterol, HDL- cholesterol and triglycerides levels at the end of the experiment (28 days) as compared to dry diet (control I). Species of lactobacilli occurring in intestinal tract deconjugate both taurocholic and glycocholic acids, such serum cholesterol levels when it is considered that deconjugated bile acids function more poorly in supporting adsorption of lipids from the intestinal tract than deconjugated ones, this could result in reduce adsorption of cholesterol from the intestines and thus influence its serum level. Therefore, the main target of the present investigation was to isolate and identify some local isolates belonging to genera Lactobacillus. Also, the isolated strains have been screened in order to define their characteristics that would be as probiotic strains or not. Furthermore, the long-term goal of this work is to registering patent protection for some Lactobacillus spp. isolated from local Egyptian resources to increase the additive values of the Egyptian microbial wealth and well use it in the industrial healthy dairy products and pharmaceutical.